Following statements are regarding to mechanism of kinesin-1 movement.

A. Initially leading head tightly bound to the microtubule and not bound by any nucleotide, while the trailing head is weakly bound to the microtubule and has ADP bound.

B. Binding of ATP induces a conformational change causing the linker to swing forward and dock into the head. This motion swings the former trailing head to become the leading head.

C. Binding of ATP induces a conformational change causing the linker to swing forward and dock into the head. This motion swings the former leading head to become the trailing head.

D. When leading head releases ADP and coordinately the trailing head hydrolyzes ATP to ADP + Pi. Pi is released and the linker becomes undocked.

E. When trailing head releases ADP and coordinately the leading head hydrolyzes ATP to ADP + Pi. Pi is released and the linker becomes undocked.

Which of the following combination is correct?

TLS Online TPP Program

#Question id: 18616

#Unit 13. Methods in Biology

In a PCR higher annealing temperature along with7-dcaza guanosine triphosphate (7-deaza OTP) used in place of GTP this indicate

TLS Online TPP Program

#Question id: 18617

#Unit 13. Methods in Biology

Both the metal ion used in PCR have different functions

TLS Online TPP Program

#Question id: 18618

#Unit 13. Methods in Biology

qPCR plot are drawn between......................, and the shape of graph is...................

TLS Online TPP Program

#Question id: 18619

#Unit 13. Methods in Biology

which of the following will give not give fluorescence

TLS Online TPP Program

#Question id: 18620

#Unit 13. Methods in Biology

Variants of PCR has been devised for

a) efficiency

b) sequence specific amplification

c) mutation analysis

d) fluorescence

TLS Online TPP Program

#Question id: 18621

#Unit 13. Methods in Biology

Variants of PCR has been devised to enhance efficiency are

a) qPCR with SYBR, TaqMan and MB

b) nested PCR

c) Touchdown start PCR

d) hot start PCR