How would transcription of the E. coli trp operon be affected,

I. If Removing sequence 4;

II. Changing several nucleotides in sequence 3 so that it can base-pair with sequence 4 but not with sequence 2;

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#Question id: 19865

#Unit 13. Methods in Biology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

COLUMN I	COLUMN II
A) Enhance efficiency	i) Multiplex PCR
B) Sequence specific amplification	ii) Hot-start PCR
C) Mutational analysis	iii) Megaprimer PCR
	iv) qPCR with SYBR
	v) AS-PCR
	vi) RLM-RACE

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#Question id: 19866

#Unit 13. Methods in Biology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?

TLS Online TPP Program

#Question id: 19867

#Unit 13. Methods in Biology

Which of the following variants of PCR were devised to prevent non-specific amplification at low temperature?

TLS Online TPP Program

#Question id: 19868

#Unit 13. Methods in Biology

Which one of the following statements about FRET is correct?

a) FRET is possible only when the distance between donor and acceptor are within 1 to 10 A0

b) For FRET, GFP chromophore contain tripeptide sequence is -Gly-Phe-Pro-

c) qPCR based on FRET

d) Efficiency of FRET is directly proportional to the sixth power of the distance between donor and acceptor. 

TLS Online TPP Program

#Question id: 19869

#Unit 13. Methods in Biology

Trinucleotide repeats SSR within the gene coding region causing disease called

TLS Online TPP Program

#Question id: 19870

#Unit 13. Methods in Biology

Frequency of recognition site of the genome is depends on the __