Use the figure to answer the following question. A space probe returns with a culture of a microorganism found on a distant planet. Analysis shows that it is a carbon-based life-form that has DNA. You grow the cells in 15N medium for several generations and then transfer them to 14N medium. Which pattern in the figure would you expect if the DNA was replicated in a conservative manner?

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#Question id: 15274

#Unit 13. Methods in Biology

Eukaryotic mRNA species are isolated by using

TLS Online TPP Program

#Question id: 15275

#Unit 13. Methods in Biology

Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use

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#Question id: 15276

#Unit 13. Methods in Biology

At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

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#Question id: 15277

#Unit 13. Methods in Biology

Although PCR and replication show resemblances, but the basic difference is

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#Question id: 15278

#Unit 13. Methods in Biology

A DNA sample having mass 1 ng undergoes PCR, the eluted DNA is weighted about 1 microgram, the minimum number of cycles required

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#Question id: 15376

#Unit 13. Methods in Biology

If a protein made up of three subunit α₂βγ (Mr. of α = 25000, β = 50000, γ = 75000) which of the following correctly representing SDS and Native (buffer) gel