#Question id: 18873
#Unit 9. Diversity of Life Forms
#Question id: 33439
#Unit 1. Molecules and their Interaction Relevant to Biology
#Question id: 1668
#Unit 4. Cell Communication and Cell Signaling
What does the cytoplasmic region of cell surface IgM consists of?
#Question id: 2909
#Unit 2. Cellular Organization
The DNA can be labeled and used to probe a microarray, yielding a map of genomic sequences to which those particular nucleosomes bind. Because microarrays are often referred to as chips, this technique is called a ChIP-chip experiment. This experiment is designed to reveal the genomic DNA sequences to which a particular histone variant binds.
I. Chromatin is isolated from the cells and digested briefly with micrococcal nuclease (MNase).
II. A histone variant with an epitope tag is introduced into a particular cell type, where it is incorporated into nucleosomes.
III. The DNA bound by nucleosomes is protected from digestion, but the linker DNA is cleaved, releasing segments of DNA bound to one or two nucleosomes.
IV. The pattern of hybridization on the microarray reveals the DNA sequences bound by the nucleosomes with the histone variant.
V. Antibody is added, and the nucleosomes containing the epitope-tagged histone variant are selectively precipitated.
VI. The DNA in these nucleosomes is extracted from the precipitate, labeled, and used to probe a microarray representing all or part of the genomic sequences of that particular cell type.
Arrange the following statements in the correct order in which they are done.
#Question id: 19172
#Unit 13. Methods in Biology