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TLS Online TPP Program

#Question id: 3978

The 5'-terminal cap structure of eukaryotic mRNAs is a(n):

#Unit 3. Fundamental Processes

  1. 7-methylcytosine joined to the mRNA via a 2',3'-cyclic linkage.

  2.  7-methylguanosine joined to the mRNA via a 5' -> 3' diphosphate linkage.

  3. 7-methylguanosine joined to the mRNA via a 5' -> 5' triphosphate linkage.

  4. N6-methyladenosine joined to the mRNA via a 5' -> 5' phosphodiester bond.

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TLS Online TPP Program

#Question id: 2828

#Unit 2. Cellular Organization

A transcriptionally active gene compared with a transcriptionally inactive gene would be expected to

a. contain acetylated histones.           

b. contain unacetylated histones.

c. be sensitive to DNase I.                 

d. be resistant to DNase I.
TLS Online TPP Program

#Question id: 2829

#Unit 2. Cellular Organization

Scaffold-associated regions

a. are the chromosome attachment points for the mitotic spindle.

b. can insulate transcription units from each other.

c. are the points at which DNA interacts with histone proteins.

d. are found between transcription units.
TLS Online TPP Program

#Question id: 2830

#Unit 2. Cellular Organization

The NtrC protein from E. coli
a. is a σ54 RNA polymerase.             
b. stimulates transcription of the glnA gene.
c. is activated by a protein kinase called NtrB.
d. binds both to an enhancer site upstream of the glnA gene and to RNA polymerase.
e. has ATPase activity.
TLS Online TPP Program

#Question id: 2831

#Unit 2. Cellular Organization

Specific DNA control elements in promoters can

a. interact with general transcription factors.

b. interact with repressor proteins.                 

c. interact with activator proteins.

d. remain unavailable because of condensed chromatin. 
TLS Online TPP Program

#Question id: 2832

#Unit 2. Cellular Organization

Reporter genes are used to

a. express enzymes that are not easily assayed in cell extracts.

b. express enzymes that are easily assayed in cell extracts.

c. characterize DNA control elements.                     

d. characterize reporter plasmids.
TLS Online TPP Program

#Question id: 2835

#Unit 2. Cellular Organization

An enhancer

a. can be located upstream of a promoter.

b. can be located downstream of a promoter.

c. can be located a variable distance from the promoter.

d. is always located within 1 kb of the promoter.

e. can be cell-type-specific.