#Question id: 3816
#Unit 3. Fundamental Processes
The mechanism of DNA replication is studied in an E. coli replication fork. Which is a characteristic of an E. coli replication fork and a eukaryotic replication fork?
#Question id: 3817
#Unit 3. Fundamental Processes
DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.
If the mechanism of DNA replication in this bacterium were dispersive, what results would be found when the double-stranded DNA was analyzed in a CsCl gradient after two generations in 15N-medium?
#Question id: 3818
#Unit 3. Fundamental Processes
DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.
If the mechanism of DNA replication in this bacterium were semi-conservative, what results would be found when the double-stranded DNA was analyzed in a CsCl density gradient after three generations in 15N-medium?
#Question id: 3819
#Unit 3. Fundamental Processes
DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.
After one generation in 15N-medium, the DNA is denatured and analyzed in a CsCl gradient. If the single-stranded DNA forms two bands, one with heavy density and one with light density, what conclusion could be drawn about the mechanism of DNA replication in this bacterium?
#Question id: 3820
#Unit 3. Fundamental Processes
Meselson and Stahlʹs experiments involved growing E. coli on a medium containing 15N (heavy nitrogen) which increased the density of the bacterial DNA. Cells were then grown on a medium containing only 14N. DNA samples were obtained for analysis after one, two and three rounds of replication (generations) and centrifuged on a density gradient. The double-helical DNA forms bands in the gradient depending on its density. How many bands did Meselson and Stahl see after two generation of cells were grown on the 14N medium?
#Question id: 3821
#Unit 3. Fundamental Processes
Which mechanism contributes to accuracy during DNA replication?