TLS Online TPP Program

#Question id: 4406


An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding?

#Unit 3. Fundamental Processes
  1. Use an antibody to TFIIB and check for a supershift by EMSA.

  2. Complete TFIIB binding by adding TFIID.

  3. Conduct a nuclear run-off assay.

  4. Use a nuclease S1 assay

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TLS Online TPP Program

#Question id: 4506

#Unit 3. Fundamental Processes

Which process is very much similar to polyadenylation, because its takes place by the multiple protein complexes,

TLS Online TPP Program

#Question id: 4507

#Unit 3. Fundamental Processes

A group of introns that-unlike those we have considered thus far-can splice themselves out of pre-mRNA without the need for the spliceosome, they are called

TLS Online TPP Program

#Question id: 4508

#Unit 3. Fundamental Processes

Statement: In the case of group II introns, the chemistry of splicing and the RNA intermediates produced are the same as those for nuclear pre-mRNA.

 Explanations: I. The intron uses an A residue within the branch site to attack the phosphodiester bond at the boundary between its 5’ end and the end of the 5’ exon-that is, at the 5’ splice site. This reaction produces the branched lariat.

II. A second reaction in which the newly freed 3’ -OH of the exon attacks the 3’splice site, releasing the intron as a lariat and fusing the 3’ and 5’ exons.

TLS Online TPP Program

#Question id: 4509

#Unit 3. Fundamental Processes

Which group of introns contain an “internal guide sequence”

TLS Online TPP Program

#Question id: 4510

#Unit 3. Fundamental Processes

Which statements are true about group I and group II introns,

A. Group I (and II) introns are not enzymes because they have a turnover number of only 1.

B. A third transesterification reaction can occur to cyclize the intron.

C. They cannot be readily converted into ribozymes.

D. It is similar to the way that the self-cleaving Hammerhead could be converted to a ribozyme by separating the active site from the substrate

TLS Online TPP Program

#Question id: 4511

#Unit 3. Fundamental Processes

The proteins are not needed for the splicing reaction itself, because