TLS Online TPP Program

#Question id: 4406


An electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region. Which of the following approaches could be used to confirm that TFIIB is indeed binding?

#Unit 3. Fundamental Processes
  1. Use an antibody to TFIIB and check for a supershift by EMSA.

  2. Complete TFIIB binding by adding TFIID.

  3. Conduct a nuclear run-off assay.

  4. Use a nuclease S1 assay