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#Question id: 4489

HSP70 gene from Drosophila. This gene, activated by heat shock, is controlled by two activators GAGA binding factor and HSF,In response to heat shock, HSF binds to specific sites at the promoter and recruits a kinase, P-TEFb (positive transcription elongation factor). What is the proper mechanism of pausing and release of Pol II ?

#Unit 3. Fundamental Processes

Second phosphorylation on Serine5 is achieved through recruitment of the kinase P-TEFb
P-TEFb is part of a super elongation complex, which releases paused Pol II from the proximal promoter.
A strong acidic activator like Gal4 is able to recruit PTEFb/SEC along with the rest of the machinery
B and C Only

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#Question id: 18904

#Unit 13. Methods in Biology

Advantages of using short half-life isotopes

TLS Online TPP Program

#Question id: 18905

#Unit 13. Methods in Biology

A Vector is a DNA molecule that has the ability to

TLS Online TPP Program

#Question id: 18906

#Unit 13. Methods in Biology

A good vector must have the following properties.

a) It should be able to replicate autonomously.

b) A vector should be ideally less than 10 kb in size

c) The vector should be easy to isolate and purify.

d) vector should have suitable marker genes that allow easy detection and/or selection of the transformed host cells.

e) it should a multiple cloning site

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#Question id: 18907

#Unit 13. Methods in Biology

When expression of the DNA insert is desired, the vector should contain at least suitable control elements,

TLS Online TPP Program

#Question id: 18908

#Unit 13. Methods in Biology

pBR 322 has/have which of the following selection marker(s)?

TLS Online TPP Program

#Question id: 18909

#Unit 13. Methods in Biology

What is the compound X-gal?