A transformation experiment is performed with a donor strain that is resistant to four drugs: W, X, Y, and Z. The recipient is sensitive to all four drugs. The treated recipient population is divided up and plated on media containing various combinations of the drugs. The table below shows the results:

Drug(s) added	Number of Colonies
None	20,000
WX	76
WY	1324
WZ	1976
XY	85
XZ	69
YZ	1564
WXY	50
WXZ	62
WYZ	1354
XYZ	48
WXYZ	53

Following observation are given

A) X gene is obviously quite distant from the other three genes

B) Y gene is obviously quite distant from the other three genes

C) The Order is three closely link gene W Z Y

D) The Order is three closely link gene W X Z

Which of the following combination is correct?

#Question id: 10483

#Unit 6. System Physiology – Plant

Given below some facts regarding the stomal responses in light condition. Which one of the following would be INCORRECT?

#Question id: 10484

#Unit 6. System Physiology – Plant

Blue light mediated activation of proton pumps which is electrogenic in nature can be measured or analyze by using which system?

#Question id: 10485

#Unit 6. System Physiology – Plant

What is the role of blue light in terms of stomatal study?

a.) Blue light activates a proton-pumping ATPase in the guard cell plasma membrane and leading to stomatal opening

b.) blue-light stimulation lowers the pH of the apoplastic space surrounding the guard cells, and generates the driving force needed for ion uptake and stomatal opening

c.) The sensitivity of guard cells to blue light increases as a function of their zeaxanthin concentration

d.) npq1 mutant opens at higher fluence rates of blue light, whereas the phot1/phot2 mutant fails to show any blue light–stimulated opening

Which one of the following combination is correct?

#Question id: 10486

#Unit 6. System Physiology – Plant

A patch clamp study shows a guard cell protoplast treated in the dark with the fungal toxin fusicoccin which is a well-characterized,

#Question id: 10487

#Unit 6. System Physiology – Plant

During the patch clamp experiments measure outward electric current in picoamps, pA at the plasma membrane of a guard cell protoplast stimulated by two points A and B showing in the graph.

What is A and B

#Question id: 10488

#Unit 6. System Physiology – Plant

Blue light activates a proton pump at the guard cell plasma membrane. The relationship between proton pumping at the guard cell plasma membrane and stomatal opening is evident from the observations that;

 a.) Orthovandate inhibits the fusicoccin-stimulated opening

 b.) fusicoccin stimulates both proton extrusion from guard cell protoplasts and stomatal opening

 c.) CCCP inhibits the fusicoccin-stimulated opening

 d.) CCCP, a proton ionophore that makes the plasma membrane highly permeable to protons resulting stomatal opening

 Which one of the following observation from the above statements should  be correct?