#Question id: 4117
#Unit 3. Fundamental Processes
The RNA recognition motif (RRM), also called the RNP motif and the RNA-binding domain (RBD), is the most common RNA-binding domain in hnRNP proteins. Choose important characteristics of RBD
a. RRM domain consists of a four-stranded b sheet flanked on one side by two a helices.
b. To interact with the negatively charged RNA phosphates, the b sheet forms a positively charged surface
c. The 45-residue KH motif is also found in the hnRNP and several other RNA-binding proteins
Which of the following statements are correct?
#Question id: 4118
#Unit 3. Fundamental Processes
If the 5’ splice site sequence changed from 5’- GUAAGU-3’ to 5’-GUAUGU-3’, predict the effect of the sequence change on U1 binding and U6 snRNP binding in an in vitro protein– RNA binding assay.
#Question id: 4119
#Unit 3. Fundamental Processes
Many eukaryotic genes contain a large number of exons. Correct splicing of such genes requires that neighboring exons be ligated to one another; if they are not, exons will be left out. One early proposal suggested that the splicing machinery bound to a splice site at one end of an intron and scanned through the intron to find the splice site at the other end. Such a scanning mechanism would guarantee that an exon was never skipped. This hypothesis was tested with one minigene with a duplicated 5ʹ splice site. Find diagram of the products you expect from minigene if the splicing machinery binds to a 5ʹ splice site and scans toward a 3ʹ splice site.
#Question id: 4120
#Unit 3. Fundamental Processes
Which of the following homolog matching is incorrectly matched with thier respective subunits?
Bacterial |
Archaea |
RNAPI |
RPAII |
RPAIII |
|
1. |
B’ |
A’/A’’ |
RPA1 |
RPB1 |
RPC1 |
2. |
B |
K |
RPA3 |
RPB2 |
RPC4 |
3. |
aI |
D |
RPC5 |
RPB3 |
RPC5 |
4. |
aII |
L |
RPC9 |
RPB11 |
RPC9 |
#Question id: 4121
#Unit 3. Fundamental Processes
The transition from the closed to the open complex involves structural changes in the enzyme and the opening of the DNA double helix to reveal the template and nontemplate strands. This “melting” involves
A. Isomerization reaction and it does not require energy derived from ATP hydrolysis
B. Isomerization is essentially irreversible and, once complete, typically guarantees that transcription will subsequently initiate
C. Positions –11 and -2, with respect to the transcription start site.
#Question id: 4122
#Unit 3. Fundamental Processes
RNA Pol uses its active site, in a simple back-reaction, to catalyze the removal of an incorrectly inserted ribonucleotide. This phenomenon known as