TLS Online TPP Program

#Question id: 5761


E. coli cells were spread on an agar plate, producing 1000 colonies.  The colonies are replica plated on two agar plates containing the antibiotic kanamycin and one agar plate without antibiotics.  All of the colonies are able to grow on the agar plate without antibiotic but only 4 colonies are able to grow on each of the agar plates containing kanamycin.  You notice that the four colonies that grew on each of the kanamycin containing plates are in the exact same position.  This demonstrates

#Unit 8. Inheritance Biology
  1. how to screen for environmental mutagens

  2. that mutations occur in prokaryotes as well as in eukaryotes

  3. that in some cases mutations are caused by the selective agent itself

  4. a direct correlation between the amount of the selective agent used and the number of resistant mutants (one-hit relationship)

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TLS Online TPP Program

#Question id: 21255

#Unit 12. Applied Biology

Industrially, the most important primary metabolites are amino acids, nucleotides and vitamins, these metabolites are manufactured by__

TLS Online TPP Program

#Question id: 21256

#Unit 12. Applied Biology

Production of l-Glutamic Acid by overproducing glutamate through some microorganism__

TLS Online TPP Program

#Question id: 21257

#Unit 12. Applied Biology

Lysine is a member of the_______________of amino acids.

TLS Online TPP Program

#Question id: 21258

#Unit 12. Applied Biology

Some classes of mutants were obtained from the parental strain as the source of genetic material for transduction:
I) A strain that are resistance to thialysine
II) A strain in which both the threonine-regulated AK and HD were resistant to feedback inhibition by threonine
III) A strain is selected for ß-hydroxynorvaline Sensitive, in which HDI was sensitive to both inhibition and repression and the threonine-regulated AK was constitutively synthesized 

TLS Online TPP Program

#Question id: 21259

#Unit 12. Applied Biology

Production of l-Threonine amino acid used for feeding pigs and poultry, Starting from l-aspartate, the pathway involves five steps catalyzed by five enzymes sequence of using enzyme is that;

TLS Online TPP Program

#Question id: 21260

#Unit 12. Applied Biology

L-Cysteine has traditionally been obtained by extracting it from acid hydrolysates of human hair and animal feathers. High levels cannot be synthesized from glucose because l-cysteine feedback inhibits the enzyme, which catalyzes one of the steps in the biosynthesis of l-cysteine. These enzyme are,