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TLS Online TPP Program
#Question id: 13064
#Unit 13. Methods in Biology
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following techniques doesn’t involve use of a secondary antibody?
TLS Online TPP Program
#Question id: 19840
#Unit 13. Methods in Biology
Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;
|
DNA Polymerase |
Sources |
|
A- Tma |
i) Pyrococcus furiosus |
|
B- Deep VentTM |
ii) Pyrococcus
woesi |
|
C- Tli |
iii) Thermotoga maritima |
|
D- Pfu |
iv) Pyrococcus
sp. |
|
E- Pwo |
v) Thermococcus litoralis |
TLS Online TPP Program
#Question id: 17202
#Unit 13. Methods in Biology
The probability that at least one of the events Q and R occur is 0.6. If Q and R have probability of occurring together as 0.2, then P(Q) + P(R) is..........
TLS Online TPP Program
#Question id: 19817
#Unit 12. Applied Biology
What is linked with external guide sequences is a potential alternative to Ribozymes
TLS Online TPP Program
#Question id: 2361
#Unit 2. Cellular Organization
______ of the cytoskeleton help maintain the shape of the cell.