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TLS Online TPP Program

#Question id: 31378

The following experimental manipulations were carried out with Xenopus embryo.
Manipulation X: Exposure to UV radiation leading to the failure of cortical rotation.
Manipulation Y: Gastrulae treated with lithium chloride.

The following statements were made with respect to the above manipulations. Which one of the following represents correct statement?

#Unit 5. Developmental Biology
  1. The phenotype obtained due to manipulation X can be rescued by injection of BMP in 1-cell embryo.
  2. Noggin mRNA will be enriched in embryos of manipulation X as compared to those of manipulation Y.
  3. Injection of cDNA for chordin into dorsal blastomeres leads to the induction of a secondary axis.
  4. If endogenous GSK3 is knocked out by a dominant-negative form of GSK3 in the ventral cells of the early embryo, a second axis forms.
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TLS Online TPP Program

#Question id: 33895

#Unit 2. Cellular Organization

Which technique can be used to separate single-stranded DNA from double-stranded DNA during reassociation studies?
TLS Online TPP Program

#Question id: 33896

#Unit 2. Cellular Organization

In hydroxyapatite column chromatography used during DNA reassociation studies, what is the principle behind the differential binding of single- and double-stranded DNA?
TLS Online TPP Program

#Question id: 33897

#Unit 2. Cellular Organization

Which of the following is correct combination
A. Translocation is an active process in which the DNA is driven into the head by an ATP independent mechanism. 
B. The genome is packaged into the empty capsid by the terminase enzyme.
C. The terminase uses cos sites in linear phage DNA by cleaving at cos sites.
TLS Online TPP Program

#Question id: 33898

#Unit 2. Cellular Organization

E. coli cell has 4,639,675 bp have a contour length of about 1.7 mm and E. coli cell is 1µm is length. What can be correctly inferred from this data.
TLS Online TPP Program

#Question id: 33899

#Unit 2. Cellular Organization

A. In E.coli, a natural closed DNA that is negatively supercoiled.
B. The intercalation of ethidium bromide first removes the negative supercoils and then introduces positive supercoils.
TLS Online TPP Program

#Question id: 33900

#Unit 2. Cellular Organization

A. Bacterial genome have many independent chromosomal domains
B. Bacterial genome is circular and have many intertangled domains 
C. Each domain consists of 40 kilobases (k2. of DNA 
D. The existence of separate domains donot permit different degrees of supercoiling to be maintained in different regions of the genome.

Find true and false