Deuterated acetyl-coenzyme A (-CD3 instead of –CH3) was used in an experiment to study the TCA cycle. Upon analysis of the metabolites, it was found that deuterium (D) was present in a wide variety of compounds; even compounds not associated with the citric acid cycle. This is due to

#Question id: 635

#Unit 1. Structure and Function of Biomolecules

The enymatic rate constant (kcat/Km) of orotidine 5ʹ-phosphate decarboxylase is 6 x 10⁷ M^-1s^-1 and the nonenzymatic rate constant (kn) is 3 x 10^-16 s^-1.  What is the value of the enzymeʹs catalytic proficiency?

#Question id: 636

#Unit 1. Structure and Function of Biomolecules

DNA polymerase contains a lysine residue that is important for binding to DNA. Mutations were found that converted this lysine to either glutamate, glycine, valine, or arginine. Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA?

#Question id: 637

#Unit 1. Structure and Function of Biomolecules

Effect of temperature on enzymatic catalysis is analysed by the rate v of a chemical reaction varies with temperature according to the Arrhenius Equation which explains the sensitivity of enzyme reactions to temperature. A plot to determination of the activation energy Ea involves

#Question id: 638

#Unit 1. Structure and Function of Biomolecules

Match the following class of enzymes with them

#Question id: 639

#Unit 1. Structure and Function of Biomolecules

Match the coenzymes in column I enzymes to class of enzymes in column II

#Question id: 640

#Unit 1. Structure and Function of Biomolecules

The data in the table above were collected for an enzyme-catalyzed reaction. The Km for this enzyme is approximately