Lysozyme is an enzyme that destroys bacterial cell walls. It does so by hydrolyzing the β(1→4) glycosidic linkages from N- acetylmuramic acid (NAM or MurNAc) to N- acetylglucosamine (NAG or GlcNAc) in cell wall peptidoglycans. The only functional groups in the immediate vicinity of lysozyme’s reactive center that have the required catalytic properties are the side chains of Glu 35 and Asp 52.

a- Glu perform reaction in its protonated state and Asp perform reaction in its deprotonated state. 

b- Glu perform reaction in its deprotonated state and Asp perform reaction in its protonated state. 

c- both in its protonated form

d- both in its deprotonated form 

Choose the correct option?

TLS Online TPP Program

#Question id: 13102

#Unit 14. Methods in Biology

 With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

 

 Why is it that having more repeats leads to a band that is higher in the gel?

TLS Online TPP Program

#Question id: 13103

#Unit 14. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

 

 

Why is it that some people show only one band?

TLS Online TPP Program

#Question id: 13104

#Unit 14. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

 

 

Why is it that some people show two bands?

TLS Online TPP Program

#Question id: 13105

#Unit 14. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

  

Given the data so far, for which of the following statement is correct

TLS Online TPP Program

#Question id: 13106

#Unit 14. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 

 

Why is it that some people only have one band?

TLS Online TPP Program

#Question id: 13107

#Unit 14. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 

 

Why is it that some people show two bands?