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TLS Online TPP Program

#Question id: 7152

The size of a cell is generally controlled by the amount of DNA in the nucleus: the more DNA, the larger the cell. What do experiments in which the amount of DNA is doubled (tetraploidy in salamanders) tell us about growth control?

#Unit 5. Developmental Biology
  1. The animals grow to a normal size, and contain only half as many cells, indicating that growth is regulated at the level of absolute size, rather than cell number.
  2. The animals grow to twice the normal size, indicating that growth is regulated at the level of the number of cells present.
  3. The animals end up with cells that are twice as big, but only half as many, resulting in an animal that is half its normal size, indicating that the number of cells is the critical determinant of growth.
  4. The animals grow to a normal size, and contain only half as many cells, indicating that growth is regulated by the total amount of DNA present in an organism.
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TLS Online TPP Program

#Question id: 18778

#Unit 13. Methods in Biology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.
TLS Online TPP Program

#Question id: 18779

#Unit 13. Methods in Biology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?
Choose whether each of the following statements is true 
a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence
b) Most antigens induce a polyclonal response. 
c) For precipitation to occur, both antigen and antibody must be multivalent.
d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests
TLS Online TPP Program

#Question id: 18780

#Unit 13. Methods in Biology

Which of the following does not participate in the formation of antigen-antibody complexes?
TLS Online TPP Program

#Question id: 18781

#Unit 13. Methods in Biology

In which of the following case a large lattice is formed?
TLS Online TPP Program

#Question id: 18782

#Unit 13. Methods in Biology

The concentration of a small amount antibodies 2 μg antibody/ml can be detected
TLS Online TPP Program

#Question id: 18783

#Unit 13. Methods in Biology

Sensitivity of various immunoassays are given match them correctly

Assay

Sensitivity∗ (g antibody/ml)

a) Mancini radial immunodiffusion

i) 2

b) Ouchterlony double immunodiffusion

ii) 20–200

c) Immunoelectrophoresis

iii) 20–200

d) Rocket electrophoresis

iv) 10–50