How can evolution select for phenotypes that postpone reproduction or sexual maturity?” There is often a trade-off between reproduction and maintenance, and in many species reproduction and senescence are closely linked. Recent studies of mice, Caenorhabditis elegans, and Drosophila suggest that there is a conserved genetic pathway that regulates aging and that it can indeed be selected for. This pathway involves the response to insulin and insulin-like growth factors, Name of these transcription factors are

TLS Online TPP Program

#Question id: 12917

#Unit 13. Methods in Biology

Arrange the steps of stages in PCR in correct order.

1. The region to be specifically amplified is made accessible within the complex DNA.

2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.

3. A thermal cycler is used to incrementally decrease the annealing temperature.

4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.

TLS Online TPP Program

#Question id: 12918

#Unit 13. Methods in Biology

Name the enzyme which helps in the DNA synthesis in the PCR.

TLS Online TPP Program

#Question id: 12919

#Unit 13. Methods in Biology

Which of the following statement is not correct about RT-PCR. 

TLS Online TPP Program

#Question id: 12920

#Unit 13. Methods in Biology

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.

TLS Online TPP Program

#Question id: 12921

#Unit 13. Methods in Biology

Match the following:-

A. Gene probe production.              i. Novel proteins

B. Genome mapping.                       ii. PCR mutagenesis

C. Protein Engineering.                 iii. Sequence tagged sites

D. Gene Editing.                                iv. Use with blots

TLS Online TPP Program

#Question id: 12922

#Unit 13. Methods in Biology

 Match the following:-

1. Ligase chain reaction.              i. Mutation detection

2. Nucleic acid sequence based   amplification.                            ii.Non isothermal                   

3. Loop mediated isothermal amplification.                      iii. Viral detection

4. Branched DNA amplification.   iv.parasitic disease