TLS Online TPP Program

#Question id: 20940


A recombinant protein is expressed in E. coli under T7 promoter at 37oC. However no biological activity is obtained in the cell lysate. If the same experiment is carried
out at 25oC, the cell lysate shows a reasonable biological activity. The most probable explanation for this is:

#Unit 13. Advances in Biotechnology
  1. lower temperature increases recombinant protein stability
  2. lower temperature increases rate of production of recombinant protein
  3. IPTG used for induction does not get degraded
  4. recombinant protein is properly folded at low temperature