Which of the following is the most logical conclusion about the distribution of the two species of barnacle, Chthamalus and Balanus?

 

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#Question id: 863

#Unit 1. Molecules and their Interaction Relevant to Biology

Ubiquitinated proteins are proteolytically degraded in an ATP-dependent process mediated by a large (∼2500 kD, 26S) multiprotein complex named the 26S proteasome;

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#Question id: 15245

#Unit 5. Developmental Biology

which of the following is property of Syncytial specification?

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#Question id: 13196

#Unit 1. Molecules and their Interaction Relevant to Biology

 In The Mica Experiment Dnase I enzyme cleaves 

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#Question id: 15273

#Unit 13. Methods in Biology

In RNA isolation, for protein free solution what will be the ratio at  nm

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#Question id: 13093

#Unit 13. Methods in Biology

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.