Each of the nine glycolytic intermediates between glucose and pyruvate is phosphorylated. The phosphoryl groups seem to have many functions such as,a) The plasma membrane generally lacks transporters for phosphorylated sugars, the phosphorylated glycolytic intermediates cannot leave the cellb) After the initial phosphorylation, further energy is necessary to retain phosphorylated intermediates in the cell, despite the large difference in their intracellular and extracellular concentrationsc) Phosphoryl groups are essential components in the enzymatic conservation of metabolic energy, Energy released in the breakage of phosphoanhydride bonds is partially conserved in the formation of phosphate esters such as glucose 6-phosphated) Binding energy resulting from the binding of phosphate groups to the active sites of enzymes lowers the activation energy and increases the specificity of the enzymatic reactionswhich of the following functions shows Importance of Phosphorylated Intermediates? -triyambak.org