#Question id: 11721

The induction of α-amylase synthesis in barley aleurone layers by gibberellin, given some statements about after the induction of GA into the aleurone layer, and allows some transcriptional gene function inside the nucleus such as;

a) GA-MYB is a negative regulator of α-amylase transcription

b) The sequence of the gibberellic acid response element (GARE) in the α-amylase gene promoter is similar to the DNA sequence to which MYB proteins bind.

c) Synthesis of GA-MYB mRNA begins to increase as early as 1 h after gibberellin treatment, preceding the increase in a-amylase mRNA by several  hours

d) A mutation in the GARE that prevents MYB binding also prevents  α-amylase expression.

e) GA-MYB can therefore be defined as a primary or early response gene, while the α-amylase gene is a secondary or late response gene

f) The secretory pathway requires GA stimulation of the calcium-calmodulin– independent pathway.

Which of the following statements is incorrect about this transcriptional gene?