A 3-week-old infant is brought to the emergency room in a comatose condition. The history reveals that her parents have been feeding her concentrated, undiluted formula for 5 days. (Infant formula preparations are often sold in concentrated forms that must be properly diluted with water before feeding.) The infant’s plasma osmolality is 352 mOsm/L (normal is 280 to 300 mOsm/L), and the osmolality of the urine is 497 mOsm/L. What is the explanation for the hyperosmotic condition of the plasma?

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#Question id: 12907

#Unit 13. Methods in Biology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____

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#Question id: 12908

#Unit 13. Methods in Biology

Which statement is incorrect about CRISPR?

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#Question id: 12909

#Unit 13. Methods in Biology

According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 12910

#Unit 13. Methods in Biology

Match the following:

A. RNA analysis               i. Sexing or cell mutation sites               a. Expressed Sequence tag

B. Forensic science           ii. mRNA analysis                                     b.  Analysis of DNA from blood

C. Gene discovery              iii. DNA profiling                                       c. Active latent viral infections

D. Single-cell analysis     iv.  RT–PCR                                             d. Sex determination of unborn

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#Question id: 12911

#Unit 13. Methods in Biology

Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

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#Question id: 12912

#Unit 13. Methods in Biology

Name the enzyme which separates the double-helical DNA into single strands.