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#Question id: 12186

In 2001, the Nobel Prize in Physiology or Medicine was awarded to three cell cycle scientists. Paul Nurse was recognized for his studies with the fission yeast S. pombe, in particular for the discovery and characterization of the wee1+ gene. What did the characterization of the wee1+ gene tell us about cell cycle control?

#Unit 2. Cellular Organization

The dual-specificity protein kinase Wee1 phosphorylates CDKs on the activatory tyrosine 15.
Yeast cells with a defective wee1+ gene activate S phase CDKs prematurely and hence experience premature entry into DNA replication.
Fission yeast cells carrying a CDK1 mutation in which the tyrosine 15 residue is replaced by phenylalanine (which is structurally similar to tyrosine but cannot be phosphorylated) show the premature mitotic CDK activation and entry into mitosis.
The dual-specificity protein phosphatase Wee1 dephosphorylates CDKs on the inhibitory tyrosine 15.

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#Question id: 18774

#Unit 13. Methods in Biology

The above Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. In these plots, r equals moles of bound ligand/mole antibody and c is the concentration of free ligand, (Ka) is slope while intercept on x axis represent

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#Question id: 18775

#Unit 13. Methods in Biology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody

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#Question id: 18776

#Unit 13. Methods in Biology

In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with

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#Question id: 18777

#Unit 13. Methods in Biology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.

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#Question id: 18778

#Unit 13. Methods in Biology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.

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#Question id: 18779

#Unit 13. Methods in Biology

Flow-cytometric analysis for the detection and measurement of subpopulations of leukocytes, including those of leukaemia, is usually performed using monoclonal antibodies. Why is this the case?

Choose whether each of the following statements is true

a) Indirect immunofluorescence is a more sensitive technique than direct immunofluorescence

b) Most antigens induce a polyclonal response.

c) For precipitation to occur, both antigen and antibody must be multivalent.

d) ELISA tests using chemiluminescence are more sensitive than chromogenic ones and precipitation tests are more sensitive than agglutination tests