Some given statements about Auxin signalling pathways are listed;

a) The auxin receptor is composed of two proteins: the SCF complex component TIR1 and the repressor protein AUX/IAA

b) The binding of auxin to its receptor complex initiates ubiquitin-dependent degradation of the AUX/ IAA repressor protein by the 26S proteasome 

c) TIR1 recruits AUX/ IAA proteins to the SCFTIR1  complex in an auxin-independent manner

d) AUX/IAA proteins are ubiquitinated by the E3 ligase activity of the SCFTIR1 complex, which marks the protein for destruction by the 26S proteasome

Which of the following is correct explanation of Auxin signalling pathways?

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#Question id: 12909

#Unit 13. Methods in Biology

According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 12910

#Unit 13. Methods in Biology

Match the following:

A. RNA analysis               i. Sexing or cell mutation sites               a. Expressed Sequence tag

B. Forensic science           ii. mRNA analysis                                     b.  Analysis of DNA from blood

C. Gene discovery              iii. DNA profiling                                       c. Active latent viral infections

D. Single-cell analysis     iv.  RT–PCR                                             d. Sex determination of unborn

TLS Online TPP Program

#Question id: 12911

#Unit 13. Methods in Biology

Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond

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#Question id: 12912

#Unit 13. Methods in Biology

Name the enzyme which separates the double-helical DNA into single strands.

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#Question id: 12913

#Unit 13. Methods in Biology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 

TLS Online TPP Program

#Question id: 12914

#Unit 13. Methods in Biology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________