TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 18610
#Unit 13. Methods in Biology
In an experiment, it was found that the value of Tm for DNA is 45 ̊C, when cell have 22% GC content at the above Tm, then what will be the effect on the value of Tm if GC% increases to 60%
TLS Online TPP Program
#Question id: 18611
#Unit 13. Methods in Biology
The long product of PCR is generated in
TLS Online TPP Program
#Question id: 18612
#Unit 13. Methods in Biology
A PCR product observed after 6 min. the product will be
a) 100% long product
b) 100% accurate product
c) 50% long product
d) 50% accurate product
TLS Online TPP Program
#Question id: 18613
#Unit 13. Methods in Biology
The size of a PCR product is mainly determined
TLS Online TPP Program
#Question id: 18614
#Unit 13. Methods in Biology
The primer length affects the efficiency rate of PCR, this rate decreases as the primer length
TLS Online TPP Program
#Question id: 18615
#Unit 13. Methods in Biology
PCR efficiency is influenced by several factors, the efficiency declines due to
a) efficiency of amplification declines with an increase in the length of the target sequence.
b) Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.
c) a temperature higher than the ideal annealing temperature reduces PCR efficiency.
d) primers used for PCR have complementary regions, dimers will be formed, and PCR efficiency will decline.