TLS Online TPP Program

#Question id: 12715


Early-acting stress sensors provide the initial signal for the stress response,  several possible examples of stress-sensing mechanisms that act early in the pathway have been identified. They include:
A) calcium channel (cyclic nucleotide-gated calcium channel) that senses changes in temperature
B) A kinase (SnRK1, a SNF1-related kinase 1) that senses energy depletion during stress
C) A plasma membrane histidine kinase (ATHK1) that senses osmotic stress
D) A leucine rich repeat receptor kinase (LRRK) protein (Srlk)
E) An endoplasmic reticulum transmembrane sensor inositol-requiring enzyme 1 (IRE1)
And activating several response after sensing these mechanisms;
i) activates hundreds of stress-response transcripts
ii) that functions as an upstream regulator of salinity responses in Arabidopsis
iii) that is required for acclimation to heat stress
iv) that is required for heat tolerance in plants
v) activates ABA-dependent and ABA-independent responses
Which of the following is the correct match the activating response with its sense mechanism;

#Unit 6. System Physiology – Plant
  1. A-iii, B-i, C-v, D-ii, E-iv
  2. A-v, B-iii, C-iv, D-i, E-ii
  3. A-ii, B-iv, C-i, D-v, E-iii
  4. A-iv, B-v, C-ii, D-iii, E-i
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TLS Online TPP Program

#Question id: 13065

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Agglutination of antigens by utilising specific antibodies can only occur, if

TLS Online TPP Program

#Question id: 13066

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is a codominant marker?

TLS Online TPP Program

#Question id: 13067

#Unit 13. Methods in Biology

The first immunoassay formats described were methods based on the ____ reaction, which is characterised either by gel formation in a liquid phase or as an opaque band in an agar plate assay.

TLS Online TPP Program

#Question id: 13068

#Unit 13. Methods in Biology

Coating antibody; which actively traps antigen

TLS Online TPP Program

#Question id: 13069

#Unit 13. Methods in Biology

A negative agglutination test may NOT indicate the

TLS Online TPP Program

#Question id: 13070

#Unit 13. Methods in Biology

Antibodies raised against an antigen from one organism will react to a greater or lesser degree with similar antigens from a related organism, depending on how many surface epitopes they share. This has given rise to a systematic method of identification of microorganisms known as______?