Practice Questions

  1. Home
  2. Free Resources
  3. Practice Questions
  4. 9503
TLS Online TPP Program

#Question id: 12721

There are the five groups of late embryogenesis abundant (LEA) proteins found in plants

Match the following groups of LEA protein with its functional information;

 Group (family name)

                           Functional information

 

I) Group 1

   (D-19 family)

 

 

a) It is an abundant protein in cotton embryos, Each putative dimer of this group may bind as many as ten inorganic phosphates and their counterions

 

 

II) Group 2

    (D-11 family)

 

b) Possibly functions in ion sequestration to protect cytosolic metabolism When LE25 is expressed in yeast, it confers salt and freezing tolerance

 

 

III) Group 3

     (D-7 family)

 

c) In tomato, a gene encoding a similar protein is expressed in response to nematode feeding

 

 

IV) Group 4

    (D-95 family)

 

d) Contains more water of hydration than typical globular proteins Overexpression confers water deficit tolerance on yeast cells

 

 

V) Group 5

    (D-113 family)

 

e) Often localized to the cytoplasm or nucleus More acidic members of the family are associated with the plasma membrane May act to stabilize macromolecules at low water potential

 

#Unit 6. System Physiology – Plant
  1. I-d,  II-e,  III-a,  IV-c,  V-b
  2. I-d,  II-e,  III-a,  IV-b,  V-c
  3. I-e,  II-d,  III-a,  IV-b,  V-c
  4. I-c,  II-a,  III-e,  IV-b,  V-d
More Questions
TLS Online TPP Program

#Question id: 13078

#Unit 13. Methods in Biology

RFLPs have several unique advantages except this one;
TLS Online TPP Program

#Question id: 13079

#Unit 13. Methods in Biology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,
TLS Online TPP Program

#Question id: 13080

#Unit 13. Methods in Biology

In RAPDs amplification will take place only of those regions of the genome that have the 
TLS Online TPP Program

#Question id: 13081

#Unit 13. Methods in Biology

Correct statements about RAPD’s
A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.
B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,
C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.
D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.
TLS Online TPP Program

#Question id: 13082

#Unit 13. Methods in Biology

Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.
TLS Online TPP Program

#Question id: 13083

#Unit 13. Methods in Biology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,