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TLS Online TPP Program
#Question id: 12909
#Unit 13. Methods in Biology
According to the diagram, arrange the steps in correct order:
1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands.
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows.
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.
TLS Online TPP Program
#Question id: 12910
#Unit 13. Methods in Biology
Match the following:
A. RNA analysis i. Sexing or cell mutation sites a. Expressed Sequence tag
B. Forensic science ii. mRNA analysis b. Analysis of DNA from blood
C. Gene discovery iii. DNA profiling c. Active latent viral infections
D. Single-cell analysis iv. RT–PCR d. Sex determination of unborn
TLS Online TPP Program
#Question id: 12911
#Unit 13. Methods in Biology
Match the following:
A. DNA polymerases i. Alkaline phosphatase a.RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase ii. T4 DNA ligase b. Thermostable DNA polymerase used in PCR
C. Ligases iii. AMV-RT c. Removes 5′-phosphates from DNA and RNA
D. Phosphatases iv. Taq DNA pol d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond
TLS Online TPP Program
#Question id: 12912
#Unit 13. Methods in Biology
Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program
#Question id: 12913
#Unit 13. Methods in Biology
________ involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination.
TLS Online TPP Program
#Question id: 12914
#Unit 13. Methods in Biology
In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________