Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

TLS Online TPP Program

#Question id: 18937

#Unit 13. Methods in Biology

Cloning techniques can be used not only to overproduce proteins but to produce protein products altered, subtly or dramatically, from their native forms. Specific amino acids may be replaced individually by 

TLS Online TPP Program

#Question id: 18938

#Unit 13. Methods in Biology

If the sequence of at least the end portions of a DNA segment of interest is known, the number of copies of that DNA segment can be hugely amplified with the 

TLS Online TPP Program

#Question id: 18939

#Unit 13. Methods in Biology

If a DNA sequence is present in higher than usual amounts in a sample can be analysed using pcr

TLS Online TPP Program

#Question id: 18940

#Unit 13. Methods in Biology

which of the following permits the amplification of chosen segments of DNA or RNA for detailed study or cloning.

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#Question id: 18941

#Unit 13. Methods in Biology

With the construction of cDNA libraries in which each gene is fused to an epitope tag, investigators can precipitate the protein product of a gene by complexing it with the antibody that binds the epitope, a process called 

TLS Online TPP Program

#Question id: 18942

#Unit 13. Methods in Biology

Bacterial artificial chromosomes (BACs), cosmids, phages, plasmids and yeast artificial chromosomes (YACs) are all commonly used cloning vectors that differ in their cloning capacities, with a range from approximately 100 bp to 1000 kb. Which of the following is the increasing cloning capacity?