Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

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#Question id: 550

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following statements is false?

TLS Online TPP Program

#Question id: 10479

#Unit 1. Molecules and their Interaction Relevant to Biology

All hydrophobic amino acids (valine, leucine, isoleucine, etc.) share which of the following properties

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#Question id: 12741

#Unit 13. Methods in Biology

 What will happen if too much antibody is present in the Agglutination process?

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#Question id: 31068

#Unit 2. Cellular Organization

Which of the following regulatory events ensure that CDKs are active only at the appropriate time and cell cycle stage?

a) cyclin binding

b) Phosphorylation on CDK

c) CKIs

d) Phosphorylation on cyclin

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#Question id: 1295

#Unit 4. Cell Communication and Cell Signaling

What signal is released by endothelial cells to recruit circulating leukocytes?