TLS Online TPP Program

#Question id: 13063


Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the statement is worng? Aminopetrin………s

#Unit 13. Methods in Biology
  1. Inhibits Salvage pathway
  2. Inhibits De novo pathway
  3. Competes for folate binding sites on dihydrofolate reductase
  4. Blocks tetrahydrofolate synthesis