Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the statement is worng? Aminopetrin………s

TLS Online TPP Program

#Question id: 31375

#Unit 2. Cellular Organization

Which of the following pathway promote cell proliferation?

a. Mitotic exit network

b. Septation initiation network

c. Hippo pathway

d. Spindle assembly checkpoint

TLS Online TPP Program

#Question id: 31376

#Unit 2. Cellular Organization

In S. cerevisiae, DNA replication is tightly controlled, and DNA should replicate once per cell cycle. Choose the INCORRECT statement regarding why the cells do not re-replicate their DNA in the S-phase?

TLS Online TPP Program

#Question id: 31377

#Unit 2. Cellular Organization

During microfilament formation, critical concentration (Cc) represents:

TLS Online TPP Program

#Question id: 31378

#Unit 5. Developmental Biology

The following experimental manipulations were carried out with Xenopus embryo.

Manipulation X: Exposure to UV radiation leading to the failure of cortical rotation.

Manipulation Y: Gastrulae treated with lithium chloride.

The following statements were made with respect to the above manipulations. Which one of the following represents correct statement?

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#Question id: 31379

#Unit 5. Developmental Biology

The cadherin catenin complex is extremely important during compaction from a morula to the blastula. Transition of early embryonic cells into a blastula differed depending on the presence or absence of calcium ions. In addition, an investigator blocked the expression of β-catenin using vivo morpholinos to detect its effect simultaneously on compaction. Which one of the following conditions will lead to the most successful transition of the morula into the blastula?

TLS Online TPP Program

#Question id: 31380

#Unit 2. Cellular Organization

If you were to remove the ER retrieval signal from protein disulfide isomerase (PDI), which is normally a soluble resident of the ER lumen, where would you expect the modified PDI to be located?