Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 15889

#Unit 12. Applied Biology

Cell culture is used for___

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#Question id: 11825

#Unit 6. System Physiology – Plant

Ethylene receptors are localized into the______

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#Question id: 5352

#Unit 8. Inheritance Biology

Marriage occurs between individuals III-3 and III-6, what is the probability couple; son would be affected with the trait?

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#Question id: 4538

#Unit 3. Fundamental Processes

We first consider a generic case. Gene A is controlled by four signals (1, 2, 3, and 4), each working through a separate activator (activators 1, 2, 3, and 4). Gene B is controlled by three signals (3, 5, and 6), working through activators 3, 5, and 6. It shows-

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#Question id: 9570

#Unit 10. Ecological Principles

Which of the following would not be a significant component of the energy budget of a person sitting on a park bench on a warm and windy night,

exposed to the clear night sky?