Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

#Question id: 4737

#Unit 8. Inheritance Biology

Two independent pure line varieties having white and green phenotype in their fruit were cross, the all f1 progeny is white fruit. The F2 progeny obtain from selfing show white, yellow and green in ratio of 12:3:1. Which of the following is correct?

#Question id: 4738

#Unit 8. Inheritance Biology

Red kernel color in wheat results from the presence of at least one dominant allele of each of two independently segregating genes . Kernels on rr bb plants are white, and the genotypes R— bb and rr B— result in brown kernel color. Suppose that plants of a variety that is true breeding for red kernels are crossed with plants true breeding for white kernels produced F1 were allowed to selfing .what are F2 phenotype ratio ?

#Question id: 4739

#Unit 8. Inheritance Biology

Inheritance of leaf variegation in the four-o’clock plant, Mirabilis jalapa. the leaves and shoots of one variety of four-o’clock were variegated, displaying a mixture of green and white splotches. If  Flowers on the variegated branches gave rise to

#Question id: 4740

#Unit 8. Inheritance Biology

Following metabolic pathway for synthesis of anthocyanin product ? Which of the following cross produced all purple phenotype in their progeny ?

#Question id: 4741

#Unit 8. Inheritance Biology

The F2 progeny from a particular cross exhibit a modified dihybrid ratio of 9 : 7 (instead of 9 : 3 : 3 : 1). What phenotypic ratio would be expected from a testcross of the F1?

#Question id: 4742

#Unit 8. Inheritance Biology

In birds, sex is determined by a ZW chromosome scheme. Males are ZZ and females are ZW. A recessive lethal allele that causes death of the embryo is sometimes present on the Z chromosome in pigeons. What would be the sex ratio in the offspring of a cross between a male that is heterozygous for the lethal allele and a normal female?