Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 18229

#Unit 8. Inheritance Biology

You are conducting genetic linkage studies of an autosomal dominant disease. You are focused on two SSR markers that may be linked to each other and to the disease. Here is a family in which some individuals are affected:

 

Calculate LOD scores for linkage at θ = 0.1 between SSR62 and SSR93

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#Question id: 18969

#Unit 8. Inheritance Biology

Consider the ABO “blood type” gene. This single gene has three alleles called A, B, and O. There are four resultant “blood types” (phenotypic classes), as follows:C

                               

Assume Hardy-Weinberg equilibrium. In Norway, the frequencies of the A and B alleles are 0.26 and 0.07, respectively. What is the frequencies of these six genotypes (AA, AO, BB, BO, AB, and OO);

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#Question id: 18970

#Unit 8. Inheritance Biology

Consider the ABO “blood type” gene. This single gene has three alleles called A, B, and O. There are four resultant “blood types” (phenotypic classes), as follows:

                                     

Assume Hardy-Weinberg equilibrium,  the frequencies of the A and B alleles are 0.26 and 0.07, respectively. What is the frequencies of the four blood types such as A, B, AB  and O in Norway;

TLS Online TPP Program

#Question id: 19025

#Unit 8. Inheritance Biology

Consider the ABO “blood type” gene. This single gene has three alleles called A, B, and O. There are four resultant “blood types” (phenotypic classes), as follows: C

                         

Assume Hardy-Weinberg equilibrium,  the frequencies of the A and B alleles are 0.26 and 0.07, respectively. You now examine the ABO blood types in a new population. You observe that the A, B, and O blood types all have equal frequencies in this population. What are the frequencies of the A and O alleles in this population?

TLS Online TPP Program

#Question id: 19026

#Unit 8. Inheritance Biology

Consider the ABO “blood type” gene. This single gene has three alleles called A, B, and O. There are four resultant “blood types” (phenotypic classes), as follows:

                             

Assume Hardy-Weinberg equilibrium, the frequencies of the A and B alleles are 0.26 and 0.07, respectively. You now examine the ABO blood types in yet another population. You observe that the AB blood type has a frequency of 0.50 in this population. What are the frequencies of the A and O alleles in this population?

TLS Online TPP Program

#Question id: 19106

#Unit 8. Inheritance Biology

Assume that mating is random. First, consider an autosomal recessive disease that is usually lethal in childhood, and that has an incidence among newborns of 1/3000. What mutation rate would be required to maintain this frequency in the population?