Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

TLS Online TPP Program

#Question id: 210

#Unit 1. Molecules and their Interaction Relevant to Biology

Enzymes with vitamin B6 as the prosthetic group catalyze a variety of reactions involving 

TLS Online TPP Program

#Question id: 211

#Unit 1. Molecules and their Interaction Relevant to Biology

The degradation of aspartic acid to oxaloacetic acid and NH4+ is catalyzed by an enzyme with ________ as its prosthetic group.

TLS Online TPP Program

#Question id: 212

#Unit 1. Molecules and their Interaction Relevant to Biology

Which functional group is shown below?

TLS Online TPP Program

#Question id: 213

#Unit 1. Molecules and their Interaction Relevant to Biology

Which amino acid can form a covalent bond to the coenzyme pyridoxal phosphate?

TLS Online TPP Program

#Question id: 214

#Unit 1. Molecules and their Interaction Relevant to Biology

Pyridoxal phosphate is involved in which type of reaction?

TLS Online TPP Program

#Question id: 215

#Unit 1. Molecules and their Interaction Relevant to Biology

Raw egg white contains a protein called avidin. What happens if you ingest raw egg whites?