To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 4654

#Unit 11. Evolution and Behavior

Structures as different as human arms, bat wings, and dolphin flippers contain many of the same bones, these bones having developed from very similar embryonic tissues. How do biologists interpret these similarities?

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#Question id: 31231

#Unit 2. Cellular Organization

Mitotic spindle attach to chromosomes via specialized protein structures known as:

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#Question id: 4289

#Unit 3. Fundamental Processes

Match the Following

A. fidelity of codon recognition	1. involves two adjacent adenine residues in the 16S rRNA component located within the A-site of the small subunit. These bases form hydrogen bonds with the minor groove of each correct base pair formed between the anticodon and the first two bases of the codon in the A-site
B. kinetic selectivity	2. Even a single mismatch in the codon–anticodon base pairing alters the position of EF-Tu, reducing its ability to interact with the factor-binding center. This, in turn, leads to a dramatic reduction in EF-Tu GTPase activity
C. Accommodation	3. When the charged tRNA is first introduced into the A-site in a complex with EF-Tu-GTP, its 30 end is distant from the site of peptide-bond formation. To participate successfully in the peptidyl transferase reaction, the tRNA must rotate into the peptidyl transferase center of the large subunit in a process

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#Question id: 32230

#Unit 2. Cellular Organization

A preparation of nerve terminals, which undergo extensive endocytosis, was lysed by treatment with distilled water and incubated with GTP-γ-S, a nonhydrolyzable derivative of GTP. What would be the outcome?

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#Question id: 18990

#Unit 13. Methods in Biology

A list of reporter or marker genes used in gene transfers in animal cells with reporter gene and their source match them correctly

I - Chloramphenicol acetyltransferase (CAT)	A - Herpes simplex virus (HSY)
II - Luciferase (lux)	B - Pacific jellyfish (Aequoria victoria)
III - Green fluorescent protein (GFP)	C - Bacteria, firefly
IV - Thymidine kinase (Tk)	D - E. coli transposon Tn9