To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 12945

#Unit 13. Methods in Biology

It is a PCR based multiplex assay that allows a number of target sites such as deletions, duplications, mutations to be amplified with a pair of primer containing probes.

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#Question id: 12946

#Unit 13. Methods in Biology

Choose the incorrect statement for the Multiple Ligation dependent probe amplification.

TLS Online TPP Program

#Question id: 12947

#Unit 13. Methods in Biology

Match the following-

A. G- proteins.                    1.src

B. Growth factors.            2.erbB

C. Protein kinases.           3. N-ras

D. Growth factor receptor.   4.hst

TLS Online TPP Program

#Question id: 12948

#Unit 13. Methods in Biology

Proteolytic cleavage of DNA polymerase by subtilisin caused loss of its nick translation activity. Which of the following properties is lost?

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#Question id: 13023

#Unit 13. Methods in Biology

Which of the following enzymes are required for end labelling?

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#Question id: 13024

#Unit 13. Methods in Biology

Inset and the vector can be ligated if they are digested by a pair of –