#Question id: 10459
#Unit 6. System Physiology – Plant
The role of N-terminus and the C-terminus region of the cryptochrome is given below;
a) N-terminal region is involved in the dimerization of cryptochrome and conformational changes
b) C terminus of cryptochrome forms a complex with SPA1 and COP1 that prevents them from acting, thereby preventing the degradation of HY5
c) N-terminal region of cryptochrome forms a complex with SPA1 and COP1 that prevents them from acting, thereby preventing the degradation of HY5
d) Arabidopsis plants overexpressing only the C-terminal region of cryptochrome (CCT) which resemble light-grown seedlings when grown in darkness
Which of the following combinations from the above statements is correct?
#Question id: 10460
#Unit 6. System Physiology – Plant
CCT overexpression mutant shows____
#Question id: 10461
#Unit 6. System Physiology – Plant
Model of cry1 interactions with COP1/SPA1 in the regulation of photomorphogenesis, what will happen in the dark as well as in the light in wild type condition of cryptochrome?
a) In the dark, COP1/SPA1 acts to degrade transcription factors such as HY5, which is required for photomorphogenesis
b) In the dark, cry1 is activated by phosphorylation and forms a complex with COP1 and SPA1 via the C-terminal domain, preventing them from degrading protein HY5
c) In the light, COP1/SPA1 acts to degrade transcription factors such as HY5, which is required for photomorphogenesis
d) In the light, cry1 is activated directly by blue light and indirectly by blue light induced phosphorylation and forms a complex with COP1 and SPA1 via the C-terminal domain, preventing them from degrading protein HY5
e) In light as well as in dark both case cryptochrome perfom same function such as forming a complex with COP1/ SPA1 thereby promoting an increase in HY5 protein levels and transcription
Which of the following combinations from the above statements of cryptochrome is incorrect?
#Question id: 10462
#Unit 6. System Physiology – Plant
During an experiments mutating the conserved cysteine in the LOV1 domain of phot1 does not affect phototropic responsiveness (the seedlings bend toward blue light) whereas the equivalent mutation in LOV2 abolishes the response. What will be the conclusion has been drawn from this experiments?
a) LOV1 domain is secondarily responsible (primarily responsible is LOV2 domain) for kinase activation and LOV1 is not is coupled with the protein region Jα-helix that’s why it does not affect phototropic responsiveness
b) These studies have demonstrated that LOV2 domain is primarily responsible for kinase activation in response to blue light
c) This is due in part to the position of LOV2 within the phototropin molecule, where it is coupled to a protein region known as the Jα-helix
d) The function of LOV2 domain is thought to play a role in receptor dimerization by accomplishing the kinase activation in response to blue light
Which of the correct conclusion drawn from the above experiment?
#Question id: 10463
#Unit 6. System Physiology – Plant
LOV1 and LOV2, each binding a chromophore flavin mononucleotide (FMN), which one of the following statements shows incorrect prediction about LOV domains?
#Question id: 10464
#Unit 6. System Physiology – Plant
As with cryptochrome and phytochrome, the N-terminal photosensory region of the phototropins controls the activity of the C-terminal half of the protein, which contains a serine/threonine kinase domain. Following are some statements about photosensory region of the phototropins.
a) The activation of the C-terminal kinase domain then leads to receptor autophosphorylation on multiple serine residues that is required for all phototropin-mediated responses
b) In the dark, the N-terminal region, including the LOV domains, “cages” and inhibits the activity of the kinase domain
c) In the light, the N-terminal region, including the LOV domains, “cages” and inhibits the activity of the kinase domain
d) Absorption of blue photons by the LOV domains results in primary photochemical changes that lead to the uncaging of the kinase domain and its activation by the unfolding of the Jα-helix
Which one of the following combination of above statements is correct?