Practice Questions

TLS Online TPP Program

#Question id: 13162

In the amoeba Dictyostelium discoideum, cAMP acts as an extracellular signalling molecule and binds to and signals via a G protein–coupled receptor; it is not a second messenger. Amoeba cells were transfected with genes encoding two fusion proteins: a Gα fused to cyan fluorescent protein (CFP), and a Gβ fused to yellow fluorescent protein (YFP). CFP normally fluoresces 490-nm light; YFP, 527-nm light. In which conditions fluorescence
energy transfer occur from CFP to YFP?

#Unit 4. Cell Communication and Cell Signaling

If ligand binding occur it leads to association of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.
If ligand binding occur it leads to dissociation of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.
If ligand binding doesn’t occur (In resting state) it leads to dissociation of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.
If ligand binding doesn’t occur (In resting state) it leads to association of the Gα and Gβγ subunits, then fluorescence energy transfer can occur.