Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:

A. Enzymatic digestion of cell wall using cellulase and pectinase

 B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods

 C. Surface sterilization and epidermis removal from leaf explant

 D. Density gradient centrifugation to separate viable protoplasts

 E. Callus formation followed by regeneration of a complete plant

Choose the correct order:

TLS Online TPP Program

#Question id: 37848

#Plant Biotechnology

Consider the following statements about plant tissue culture techniques and their applications:

(A) Meristem culture is used for virus-free plant production.

(B) Seed culture increases efficiency of seed germination.

(C) Callus culture is used to produce somatic embryos.

(D) Anther culture and ovary culture both produce haploid plants.

(E) Suspension culture is mainly used for producing multicellular tissue masses.

(F) Protoplast culture is applied for somatic hybrid production.

Which of the following combinations of statements is correct?

TLS Online TPP Program

#Question id: 37849

#Plant Biotechnology

Column A	Column B
1. Meristem culture	A. Produce somatic embryos
2. Seed culture	B. Single cell production
3. Callus culture	C. Virus-free plant production
4. Anther culture	D. Haploid plant production
5. Suspension culture	E. Increase efficiency of seed germination
6. Protoplast culture	F. Somatic hybrid production
7. Ovary culture	G. Haploid plant production

TLS Online TPP Program

#Question id: 37850

#Plant Biotechnology

Which of the following processes describes the change of mature, differentiated plant cells into meristematic cells in culture?

TLS Online TPP Program

#Question id: 37851

#Plant Biotechnology

An unorganized mass of cells formed when plant tissues are cultured on agar-gelled nutrient medium is called:

TLS Online TPP Program

#Question id: 37852

#Plant Biotechnology

Why do callus cultures need to be subcultured every 3–5 weeks?

TLS Online TPP Program

#Question id: 37853

#Plant Biotechnology

Which of the following cell types can undergo dedifferentiation in suitable culture conditions?