Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:

A. Enzymatic digestion of cell wall using cellulase and pectinase

 B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods

 C. Surface sterilization and epidermis removal from leaf explant

 D. Density gradient centrifugation to separate viable protoplasts

 E. Callus formation followed by regeneration of a complete plant

Choose the correct order:

#Question id: 10362

#Environmental Science

Bacteria can convert atmospheric nitrogen into ammonia, Several form symbiotic associations with higher plants is given below;

               HOST PLANT                                                                          N-FIXING SYMBIONTS

A) Gunnera                                                                            i) Frankia

      B) Azolla                                                                                ii) Acetobacter

C) Leguminous                                                                     iii) Azospirillum

             D) Actinorhizal                                                                      iv) Nostoc

             E) Sugarcane                                                                         v) Anabaena

             F) Miscanthus                                                                       vi) Sinorhizobium

Which of the following combination with the host plants and n-fixing symbionts is CORRECT?

#Question id: 10363

#Botany

Free living nitrogen fixing takes place as shown in the following;

             TYPE                                                                                       N-FIXING GENERA

A) Cyanobacteria (blue-green algae)                                        i) Bacillus

B) Aerobic                                                                                     ii) Clostridium

C) Facultative                                                                              iii) Anabaena

D) Anaerobic nonphotosynthetic                                            iv) Rhodospirillum

E) Anaerobic photosynthetic                                                     v) Azospirillum

 Which of the following combination of free living nitrogen fixing bacteria is correct?

#Question id: 10363

#Plant Biotechnology

Free living nitrogen fixing takes place as shown in the following;

             TYPE                                                                                       N-FIXING GENERA

A) Cyanobacteria (blue-green algae)                                        i) Bacillus

B) Aerobic                                                                                     ii) Clostridium

C) Facultative                                                                              iii) Anabaena

D) Anaerobic nonphotosynthetic                                            iv) Rhodospirillum

E) Anaerobic photosynthetic                                                     v) Azospirillum

 Which of the following combination of free living nitrogen fixing bacteria is correct?

#Question id: 10363

#Life Sciences

Free living nitrogen fixing takes place as shown in the following;

             TYPE                                                                                       N-FIXING GENERA

A) Cyanobacteria (blue-green algae)                                        i) Bacillus

B) Aerobic                                                                                     ii) Clostridium

C) Facultative                                                                              iii) Anabaena

D) Anaerobic nonphotosynthetic                                            iv) Rhodospirillum

E) Anaerobic photosynthetic                                                     v) Azospirillum

 Which of the following combination of free living nitrogen fixing bacteria is correct?

#Question id: 10363

#Applied Microbiology

Free living nitrogen fixing takes place as shown in the following;

             TYPE                                                                                       N-FIXING GENERA

A) Cyanobacteria (blue-green algae)                                        i) Bacillus

B) Aerobic                                                                                     ii) Clostridium

C) Facultative                                                                              iii) Anabaena

D) Anaerobic nonphotosynthetic                                            iv) Rhodospirillum

E) Anaerobic photosynthetic                                                     v) Azospirillum

 Which of the following combination of free living nitrogen fixing bacteria is correct?

#Question id: 10363

#Bio-Informatics

Free living nitrogen fixing takes place as shown in the following;

             TYPE                                                                                       N-FIXING GENERA

A) Cyanobacteria (blue-green algae)                                        i) Bacillus

B) Aerobic                                                                                     ii) Clostridium

C) Facultative                                                                              iii) Anabaena

D) Anaerobic nonphotosynthetic                                            iv) Rhodospirillum

E) Anaerobic photosynthetic                                                     v) Azospirillum

 Which of the following combination of free living nitrogen fixing bacteria is correct?