Arrange the following steps involved in enzymatic isolation, culture, and regeneration of plant protoplasts in the correct sequence:

A. Enzymatic digestion of cell wall using cellulase and pectinase

 B. Culturing of isolated protoplasts by hanging drop / feeder layer / agar methods

 C. Surface sterilization and epidermis removal from leaf explant

 D. Density gradient centrifugation to separate viable protoplasts

 E. Callus formation followed by regeneration of a complete plant

Choose the correct order:

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#Question id: 34147

#Biochemistry

Which of the following statements not CORRECT regarding the mechanism and reproductive role of prolactin during lactation?

TLS Online TPP Program

#Question id: 34148

#Biochemistry

Assertion (A): Prolactin stimulates the synthesis of casein and lactalbumin during lactation without acting directly on the cell nucleus.

Reason (R): The action of prolactin is dependent on intact microtubules, suggesting involvement of cytoplasmic transport or signaling components.

Choose the correct option:

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#Question id: 34154

#Biochemistry

Which organelle is particularly abundant in adrenal cortex cells due to its role in steroid hormone synthesis?

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#Question id: 34163

#Biochemistry

Which of the following represents the correct sequential pathway for aldosterone synthesis from cholesterol, based on the enzyme-catalyzed steps?

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#Question id: 34167

#Biochemistry

I. The adrenal medulla does not regenerate, but when the inner two zones of the cortex are removed, a new ZF and ZR regenerate from glomerular cells attached to the capsule.

II. Immediately after hypophysectomy, the ZF and ZR begin to atrophy, whereas the ZG is unchanged because of the action of angiotensin II on this zone. 

III. The ability to secrete aldosterone and conserve Na+ is normal for some time after hypophysectomy, but in longstanding hypopituitarism, aldosterone deficiency may develop, apparently because of the absence of a pituitary factor that maintains the responsiveness of the zona glomerulosa. 

TLS Online TPP Program

#Question id: 37829

#Plant Biotechnology

Assertion (A): Plant tissue culture is possible because even highly mature plant cells can dedifferentiate into meristematic cells.

Reason (R): Mature animal cells cannot change back into a proliferative state once they are fully differentiated.