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TLS Online TPP Program

#Question id: 23696

which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
1. The primers hybridize to the target DNA.
2. The mixture is heated to a high temperature to denature the double stranded target DNA.
3. Fresh DNA polymerase is added.
4. DNA polymerase extends the primers to make a copy of the target DNA.

#Applied Microbiology
  1. 2, 1, 4
  2. 1, 3, 2, 4
  3. 3, 4, 1, 2
  4. 3, 4, 2
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TLS Online TPP Program

#Question id: 40463

#Applied Microbiology

  • Assertion (A): In the presence of a high concentration of an attractant, the frequency of bacterial tumbling decreases (longer runs).

  • Reason (R): Attractant binding causes a conformational change in the receptor that inhibits CheA kinase activity, leading to reduced levels of CheY-P.

    •
    TLS Online TPP Program

    #Question id: 40464

    #Applied Microbiology

    Match the Che protein in Column I with its primary function in Column II.

    Column I (Protein)Column II (Function)
    A. CheA1. Response regulator that controls motor switch
    B. CheY2. Phosphatase that dephosphorylates CheY
    C. CheZ3. Sensor Histidine Kinase
    D. CheR4. Constitutive Methyltransferase
    TLS Online TPP Program

    #Question id: 40465

    #Applied Microbiology

    During the Penetration phase of a typical T-even bacteriophage infection, which of the following events occurs?
    TLS Online TPP Program

    #Question id: 40466

    #Applied Microbiology

    Consider the following statements regarding the Biosynthesis (Synthesis) phase:

      Statement I: "Early proteins" synthesized during viral infection are typically enzymes needed for viral DNA replication and to halt host cell metabolism

      Statement II: "Late proteins" include structural components like capsid proteins and tail fibers, which are synthesized after the viral genome has been replicated.

      Which of the above statements is/are correct?
      TLS Online TPP Program

      #Question id: 40467

      #Applied Microbiology

        Assertion (A): The time interval between the penetration of the viral DNA and the release of new virions is known as the "Eclipse Period."

          Reason (R): During the eclipse period, complete infectious virus particles are not yet detected within the host cell because they are still being assembled from separate components.

          Choose the correct option:
          TLS Online TPP Program

          #Question id: 40468

          #Applied Microbiology

          Match the Viral Cycle Phase in Column I with the key molecular event in Column II.

          Column I (Phase)Column II (Key Event)
          A. Attachment1. Interaction of tail fibers with specific host cell wall receptors
          B. Release2. Action of Lysozyme/Endolysin to break the cell wall
          C. Lysogeny3. Integration of viral DNA into the host genome (Prophage)
          D. Maturation4. Spontaneous self-assembly of capsid heads and tails