TLS Online TPP Program

#Question id: 4656


Logically, which of these should cast the most doubt on the relationships depicted by an evolutionary tree?

#Zoology
  1. None of the organisms depicted by the tree ate the same foods.

  2. Some of the organisms depicted by the tree had lived in different habitats.

  3. The skeletal remains of the organisms depicted by the tree were incomplete (i.e., some bones were missing).

  4. Relationships between DNA sequences among the species did not match relationships between skeletal patterns.

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TLS Online TPP Program

#Question id: 38744

#Plant Biotechnology

Match the following enzymes commonly used in plant tissue maceration with their sources:

List IList II
A. Zymolyase1. Rhizopus arrhizus
B. Pectolyase2. Arthrobacter luteus
C. Macerase3. Aspergillus japonicus
D. Macerozyme R-104. Helix pomatia
Select the correct option:

TLS Online TPP Program

#Question id: 38745

#Plant Biotechnology

Q: Assertion (A): An osmoticum must be added to the medium during the isolation of protoplasts.
Reason (R): Protoplasts lack a cell wall and are prone to bursting without osmotic stabilization.

TLS Online TPP Program

#Question id: 38747

#Plant Biotechnology

Q. Assertion (A): Co-culturing is a vital technique for the regeneration of recalcitrant protoplasts. 
Reason (R): Fast-growing protoplasts in the medium secrete diffusible substances that stimulate cell wall synthesis in neighboring cells.

TLS Online TPP Program

#Question id: 38748

#Plant Biotechnology

Q. Assertion (A): In a co-culture system, the two types of protoplasts must be in direct physical contact to exchange nutrients.
 Reason (R): Growth factors provided by "nurse" cells are diffusible and can move through the liquid or semi-solid culture medium.

TLS Online TPP Program

#Question id: 38749

#Plant Biotechnology

Q. Assertion (A): Protoplasts are initially fragile and cannot undergo mitosis immediately after isolation. 
Reason (R): The enzymatic degradation of the cell wall during isolation removes the structural constraint required for organized cell division.

TLS Online TPP Program

#Question id: 38751

#Plant Biotechnology

Q.Assertion (A): Fast-growing protoplasts act as a "feeder layer" in co-culture experiments. 
Reason (R): They compete with slow-growing protoplasts for limited carbon sources, thereby forcing the slow-growing cells to adapt faster.