TLS Online TPP Program

#Question id: 470


If glucose labeled with 14C in C-1 were fed to yeast carrying out the ethanol fermentation, where would the 14C label be in the products?

#Applied Microbiology
  1. In C-1 of ethanol and CO2

  2. In C-1 of ethanol only

  3. In C-2 (methyl group) of ethanol only

  4. In C-2 of ethanol and CO2

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TLS Online TPP Program

#Question id: 38747

#Plant Biotechnology

Q. Assertion (A): Co-culturing is a vital technique for the regeneration of recalcitrant protoplasts. 
Reason (R): Fast-growing protoplasts in the medium secrete diffusible substances that stimulate cell wall synthesis in neighboring cells.

TLS Online TPP Program

#Question id: 38748

#Plant Biotechnology

Q. Assertion (A): In a co-culture system, the two types of protoplasts must be in direct physical contact to exchange nutrients.
 Reason (R): Growth factors provided by "nurse" cells are diffusible and can move through the liquid or semi-solid culture medium.

TLS Online TPP Program

#Question id: 38749

#Plant Biotechnology

Q. Assertion (A): Protoplasts are initially fragile and cannot undergo mitosis immediately after isolation. 
Reason (R): The enzymatic degradation of the cell wall during isolation removes the structural constraint required for organized cell division.

TLS Online TPP Program

#Question id: 38751

#Plant Biotechnology

Q.Assertion (A): Fast-growing protoplasts act as a "feeder layer" in co-culture experiments. 
Reason (R): They compete with slow-growing protoplasts for limited carbon sources, thereby forcing the slow-growing cells to adapt faster.

TLS Online TPP Program

#Question id: 38753

#Plant Biotechnology

Q. Which staining method is used specifically to detect the onset of cell wall formation in cultured protoplasts? 

TLS Online TPP Program

#Question id: 38754

#Plant Biotechnology

Q. A researcher observes protoplasts under a fluorescence microscope and sees a bright yellow-green glow. This indicates that: