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TLS Online TPP Program

#Question id: 18625

m.RNA variation can be analyzed by using

#Part-A Aptitude & General Biotechnology
  1. Touchdown PCR
  2. nested PCR
  3. RACE
  4. Overlap extension PCR
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TLS Online TPP Program

#Question id: 12960

#Part-B Specialized Branches in Biotechnology

The Norway rat (Rattus Norvegicus), a widespread pest, was controlled for about a decade by the anticoagulant warfarin. This chemical substance, placed in food pellets, is absorbed by the intestinal tract and inhibits the clotting of blood. After a population decline for about 10 years, rat populations increased and stabilized. In one European population, as illustrated in the graph below, the percentage of rats resistant to warfarin has remained fairly stable over a number of years.

Resistance to warfarin is governed by a dominant autosomal gene, R. More than 15 percent of the resistant animals are heterozygous at this locus (Rr). The table below indicates the response to warfarin and relative reproductive fitness of individuals that are homozygous or heterozygous for the dominant gene (R). The RR individuals have a 20-fold increase in vitamin K requirement over individuals.

Fitness is a measure of the reproductive success of a particular genotype. The highest fitness is 1.00.
Which of the following is most likely correct concerning the gene for resistance to warfarin? 
TLS Online TPP Program

#Question id: 13023

#Part-A Aptitude & General Biotechnology

Which of the following enzymes are required for end labelling?
TLS Online TPP Program

#Question id: 13030

#Part-A Aptitude & General Biotechnology

The mutation in Vir F will cause the interference in –
TLS Online TPP Program

#Question id: 13034

#Part-A Aptitude & General Biotechnology

Which the following statements is/are true about the stacking gel?
i. The stacking gel solution contains acrylamide.
ii. It is on the top of the separating gel.
iii. It is used to dilute the protein sample before it enters the main separating gel.
iv. It is into this gel that the wells are formed and the proteins loaded.
TLS Online TPP Program

#Question id: 13038

#Part-A Aptitude & General Biotechnology

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order
i. incubate with antibody-enzyme complex that binds primary antibody
ii. coat surface with antigen, block unoccupied sites with nonspecific protein
iii. add substrate, formation of colored product indicates presence of specific antigen
iv. incubate with primary antibody against specific antigen
TLS Online TPP Program

#Question id: 13039

#Part-A Aptitude & General Biotechnology

 Which of the following is incorrect about a microarray?