Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

#Question id: 10344

#Part-B Specialized Branches in Biotechnology

Some of the following caracteristics of Nitrite reductase, which one of the is incorrect?

#Question id: 10345

#Part-B Specialized Branches in Biotechnology

In Leaf Chloroplasts and root plastids contain different forms of the enzyme, nitrite reductase such as

a) Roots contain fd-dependent nitrite reductase

b) Shoots contain NADPH dependent nitrite reductase

c) Shoots contain fd-dependent nitrite reductase

d) Roots contain NADPH dependent nitrite reductase

Which one of the following is correct combination?

#Question id: 10350

#Part-B Specialized Branches in Biotechnology

Ammonium can be assimilated via an alternative pathway rather than primary pathway which involves the sequential actions of glutamine synthetase(GS) and glutamate synthase(GOGAT), these alternative pathway catalyzes by enzyme known as,

#Question id: 10352

#Part-B Specialized Branches in Biotechnology

which of the following amino acid that participates in the malate–aspartate shuttle to transfer reducing equivalents from the mitochondrion and chloroplast into the cytosol?

#Question id: 10353

#Part-B Specialized Branches in Biotechnology

Aminotransferases are found in multiple organelles such as cytoplasm, chloroplasts, mitochondria, glyoxysomes, and peroxisomes, these aminotransferases one of its organelles may have a significant role in amino acid biosynthesis such as;

#Question id: 10357

#Part-B Specialized Branches in Biotechnology

Why the glutamine and glutamate will not be transported while  asparagine will prefer to be transported?