The idea behind CARs was to engineer the T-cell receptor to trigger activation of ZAP70 in response to the presence of a tumor-specific antigen rather than the MHC peptide complex. 

TLS Online TPP Program

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#Section 3: Genetics, Cellular and Molecular Biology

Each sarcomere contains two major types of filaments: 

Thick filaments (myosin II)

Thin filaments (actin and associated proteins)

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#Section 3: Genetics, Cellular and Molecular Biology

Sarcomere

Within each skeletal muscle cell are many myofibrils consisting of a repeating array of a specialized structure

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#Section 3: Genetics, Cellular and Molecular Biology

When the Ca2+ levels drop:

Myosin LC is dephosphorylated by MLC phosphatase, causing muscle relaxation.

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#Section 3: Genetics, Cellular and Molecular Biology

Phosphorylation of MLC 

unfolds the myosin II, active and can assemble into bipolar filaments for contraction

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#Section 3: Genetics, Cellular and Molecular Biology

The CaM*-Ca2+ complex binds and activates MLC kinase, phosphorylates the myosin LC. 

TLS Online TPP Program

#Id: 8391

#Section 3: Genetics, Cellular and Molecular Biology

When the Ca2+ level rises, Ca2+ binds CaM.