The idea behind CARs was to engineer the T-cell receptor to trigger activation of ZAP70 in response to the presence of a tumor-specific antigen rather than the MHC peptide complex. 

TLS Online TPP Program

#Id: 2229

#Section 3: Genetics, Cellular and Molecular Biology

The Birth of a Gene - Duplication is the most common way that new genes arise in eukaryotes. 

TLS Online TPP Program

#Id: 8952

#Section 3: Genetics, Cellular and Molecular Biology

One is unequal crossing over, in which recombination happens between different positions on chromosomes that are misaligned during meiosis. 

Another is replication slippage, in which the DNA polymerase loses its place and copies a segment of chromosome twice.

TLS Online TPP Program

#Id: 8953

#Section 3: Genetics, Cellular and Molecular Biology

A third way that gene duplicates arise is by retrotransposition.

Here the messenger RNA from a gene is reverse-transcribed to DNA, which is then integrated into the genome.

TLS Online TPP Program

#Id: 8954

#Section 3: Genetics, Cellular and Molecular Biology

As a chromosome with a new gene duplicate spreads through a population, there is polymorphism in the number of copies of the gene that individuals carry. This situation, called copy number variation.

TLS Online TPP Program

#Id: 8955

#Section 3: Genetics, Cellular and Molecular Biology

TLS Online TPP Program

#Id: 8956

#Section 3: Genetics, Cellular and Molecular Biology

Mixtures of exons duplicated from genes with different functions can generate new genes with new functions, a process that is called exon shuffling.