Function of the ER:

most eukaryotic cells is to sequester Ca2+ from the cytosol (High concentration of Ca2+-binding proteins in the ER facilitates Ca2+ storage)

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Histidine: Two component signalling, C2H2 zinc finger, Hemoglobin, His6-NiNTA column.

Isoleucine: AATS proofreading, RTK

Leucine: Toll like receptors, LRR, 

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Detyrosylation 

tyrosine can be removed by a carboxypeptidase, where it sequentially removes the C-terminal tyrosines from α-tubulin subunits. 

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His ionisation series

1------pK1-----pKr--------pKr------14