The translocon was first identified through mutations in the yeast gene encoding a protein called Sec61α

TLS Online TPP Program

#Id: 8866

#Section 3: Genetics, Cellular and Molecular Biology

TLS Online TPP Program

#Id: 8867

#Section 3: Genetics, Cellular and Molecular Biology

Cdc25B is act as a starter phosphatase that initiates CDK1 activation

Cdc25C is the workhorse phosphatase that activates the vast majority of cyclin-CDK1 complexes

TLS Online TPP Program

#Id: 8868

#Section 3: Genetics, Cellular and Molecular Biology

TLS Online TPP Program

#Id: 8869

#Section 3: Genetics, Cellular and Molecular Biology

Yeast cells with a defective wee1+ gene activate mitotic CDKs prematurely and premature entry into mitosis (smaller than normal cells

TLS Online TPP Program

#Id: 8870

#Section 3: Genetics, Cellular and Molecular Biology

Fission yeast cells carrying a CDK1 mutation in which the tyrosine-15 residue is replaced by phenylalanine

show the same premature mitotic CDK activation and entry into mitosis.

TLS Online TPP Program

#Id: 8871

#Section 3: Genetics, Cellular and Molecular Biology

Until Mitotic CDKs is not active, the activatory protein phosphatase, Cdc25, is phosphorylated, bound and inhibited by phosphoserine/threonine-binding protein 14-3-3.