Vesicles coated with GGA proteins and clathrin bind to proteins containing 

Asp-X-Leu-Leu and Asp-Phe-Gly-X-Φ sequences

TLS Online TPP Program

#Id: 8615

#Section 3: Genetics, Cellular and Molecular Biology

Y15 & T14 are situated in the G-loop of the CDK, containing the consensus sequence GEGT14Y15GVV

TLS Online TPP Program

#Id: 8616

#Section 3: Genetics, Cellular and Molecular Biology

Phosphorylation of T14 and Y15 in this G-loop

introduces negative charge that electrostatically interferes with the binding of ATP in the catalytic pocket.

TLS Online TPP Program

#Id: 8617

#Section 3: Genetics, Cellular and Molecular Biology

Wee1 kinase Inhibits CDKs in all organisms

TLS Online TPP Program

#Id: 8618

#Section 3: Genetics, Cellular and Molecular Biology

Cdc25 phosphatase Activates CDKs in fission yeast

TLS Online TPP Program

#Id: 8619

#Section 3: Genetics, Cellular and Molecular Biology

INK4s

bind to CDK4 and CDK6 monomers, blocking their interaction with cyclin D

TLS Online TPP Program

#Id: 8620

#Section 3: Genetics, Cellular and Molecular Biology

A second class of CKIs found in metazoan cells is the Cip/Kip family of CKIs, consists of three proteins—p21CIP, p27KIP1, and p57KIP2.