After assembly of the matrix protein-Pex5-Pex14 complex, the matrix protein dissociates from Pex5 and is released into the peroxisomal matrix.

TLS Online TPP Program

#Id: 8326

#Section 3: Genetics, Cellular and Molecular Biology

The two ends of microfilament polymerize at different rates:

fast-growing end - plus end

slow-growing end - minus end

TLS Online TPP Program

#Id: 8325

#Section 3: Genetics, Cellular and Molecular Biology

Critical concentration (Cc)

minimal concentration of monomers at which filaments form

TLS Online TPP Program

#Id: 8324

#Section 3: Genetics, Cellular and Molecular Biology

The rate of addition of monomers is given by

rate constant kon (M–1 sec–1 )

The rate of loss is given by koff (sec–1)

TLS Online TPP Program

#Id: 8323

#Section 3: Genetics, Cellular and Molecular Biology

TLS Online TPP Program

#Id: 8322

#Section 3: Genetics, Cellular and Molecular Biology

Steady-state phase

G-actin monomers exchange with subunits at the filament ends, but there is no net change in the total length of filaments.

TLS Online TPP Program

#Id: 8321

#Section 3: Genetics, Cellular and Molecular Biology

Elongation phase

short oligomer rapidly increases in length by the addition of actin monomers to both of its ends, causing filament elongation