Aspartase enzyme is used industrially for manufacture of aspartic acid, a component of low-calorie sweetener. Fumaric acid and ammonia are converted to aspartic acid according to the equation:

 

                                        

Under investigation is a process using aspartase in intact Bacillus cadaveris cells. In the substrate range of interest, the conversion can be described using Michaelis-Menten kinetics with K m 4.0 gl^-1. The substrate solution contains 15% (w/v) ammonium fumarate; enzyme is added in the form of lyophilised cells and the reaction stopped when 85% of the substrate is converted. At 32⁰C vmax for the enzyme is 5.9 g l^-1h^-1 and its half-life is 10.5 d. At 37⁰C Vmax increases to 8.5 g l^{- 1} h^{- I} but the half-life is reduced to 2.3 d. The average downtime between batch reactions is 28 h. calculate the reactor volume required to produce 5000 tonnes of aspartic acid per year

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#Question id: 15700

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In a sequence database of a given size, which of the following expressions is likely to retrieve more matches (X means any amino acid; any of the residues in square brackets can occupy that position)?

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#Question id: 15701

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In a sequence logo of the type given below, the sizes of the letters are proportional to the:

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#Question id: 15702

#Section 6: Plant, Animal and Microbial Biotechnology

Following are few sterilization techniques and materials used in plant tissue culture. Identify the correct combinations.

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#Question id: 15703

#Section 6: Plant, Animal and Microbial Biotechnology

Most commonly used carbon source in nutrient medium for plant tissue culture is

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#Question id: 15715

#Section 6: Plant, Animal and Microbial Biotechnology

In embryogenesis a type of structure with no vascular connection with the maternal callus tissue or explants is formed

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#Question id: 15726

#Section 6: Plant, Animal and Microbial Biotechnology

Tissues and cells cultured in a liquid medium produce culture of single cells and cells clumps of few to many cells