Invertase is immobilised in ion-exchange resin of average diameter 1 mm. The amount of enzyme in the beads is measured by protein assay as 0.05 kg m ^-3. 20 cm ^3 beads are packed into a small column reactor; 75 ml sucrose solution at a concentration of 16 mM is pumped rapidly through the bed. In another reactor an identical quantity of free enzyme is mixed into the same volume of sucrose solution. Assume the kinetic parameters for free and immobilised enzyme are equal: K,,, is 8.8 mM and the turnover number is 2.4 x 10 gmol glucose (g enzyme)-'s^-1. The effective diffusivity of sucrose in the ion-exchange resin is 2 x 10^-6cm2s 4. What is the rate of reaction by free enzyme? 

TLS Online TPP Program

#Question id: 14735

#Section 2: General Biology

Large surface are a to volume ratio of bacterial cell give advantage for

TLS Online TPP Program

#Question id: 14736

#Section 2: General Biology

In basal structure of flagella of gram positive bacteria?

1. L

2. P

3. S

4. M

Which combination is not found in above bacteria? Choose the option with both incorrect rings.

TLS Online TPP Program

#Question id: 14737

#Section 2: General Biology

Which structures are involved in formation of biofilms

TLS Online TPP Program

#Question id: 14741

#Section 5: Bioprocess Engineering and Process Biotechnology

Microbial cell recovery is carried out in a continuous disc-stack centrifuge. The centrifuge is operated at 5000 rpm for separation of baker's yeast. At a feed rate of 60 1/min, 50% of the cells are recovered. At constant speed, solid recovery is inversely proportional to flow rate. What operating speed is required to recover 90% of cells if the flow rate in the centrifuge is maintained at 601/min? 

TLS Online TPP Program

#Question id: 14742

#Section 5: Bioprocess Engineering and Process Biotechnology

Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-⁴m³, Wr = 3x10^-3m³ /kg dry sephacryl, ps =1.25 x 10³ kg/m³ . The partition coefficients are k_pA =0.38 and k_pB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone? 

TLS Online TPP Program

#Question id: 14743

#Section 5: Bioprocess Engineering and Process Biotechnology

for the fermenter with the given data. 

Air is sparged with 0.4 vvm, it is equipped with two sets of impellers and a flat-blade turbine with four baffles. calculate power, P