Invertase is immobilised in ion-exchange resin of average diameter 1 mm. The amount of enzyme in the beads is measured by protein assay as 0.05 kg m ^-3. 20 cm ^3 beads are packed into a small column reactor; 75 ml sucrose solution at a concentration of 16 mM is pumped rapidly through the bed. In another reactor an identical quantity of free enzyme is mixed into the same volume of sucrose solution. Assume the kinetic parameters for free and immobilised enzyme are equal: K,,, is 8.8 mM and the turnover number is 2.4 x 10 gmol glucose (g enzyme)-'s^-1. The effective diffusivity of sucrose in the ion-exchange resin is 2 x 10^-6cm2s 4. What is the rate of reaction by free enzyme? 

TLS Online TPP Program

#Question id: 13109

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 

 which number of repeats did Baby C inherit from his mother?

TLS Online TPP Program

#Question id: 13110

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 

 

Match the babies up with their actual parents.

 

TLS Online TPP Program

#Question id: 13115

#Section 3: Genetics, Cellular and Molecular Biology

Match the correct one -

TLS Online TPP Program

#Question id: 13116

#Section 3: Genetics, Cellular and Molecular Biology

In chloroplast, the site of coupled oxidation-reduction reactions is the

TLS Online TPP Program

#Question id: 13123

#Section 3: Genetics, Cellular and Molecular Biology

Match the correct one?

A- COPI                           i- vesicles transport proteins from the ER to the Golgi.

B- COPII                          ii- vesicles mainly transport proteins in the retrograde direction between Golgi cisternae and from the cis -Golgi back to the ER.

C- Clathrin                      iii- vesicles transport proteins from the plasma membrane (cell  surface) and the trans-Golgi network to late endosomes.

TLS Online TPP Program

#Question id: 13153

#General Aptitude

The in-radius of an equilateral triangle is of length 3 cm. Then the length of each of its medians is