Invertase is immobilised in ion-exchange resin of average diameter 1 mm. The amount of enzyme in the beads is measured by protein assay as 0.05 kg m ^-3. 20 cm ^3 beads are packed into a small column reactor; 75 ml sucrose solution at a concentration of 16 mM is pumped rapidly through the bed. In another reactor an identical quantity of free enzyme is mixed into the same volume of sucrose solution. Assume the kinetic parameters for free and immobilised enzyme are equal: K,,, is 8.8 mM and the turnover number is 2.4 x 10 gmol glucose (g enzyme)-'s^-1. The effective diffusivity of sucrose in the ion-exchange resin is 2 x 10^-6cm2s 4. What is the rate of reaction by free enzyme? 

TLS Online TPP Program

#Question id: 19486

#Section 4: Fundamentals of Biological Engineering

The growth of S.cerevisiae on glucose under anaerobic conditions can be described by the following reaction:

C₆H₁₂O₆ + aNH₃ → 0.59 CH_1.64N_0.16O_0.52 (biomass) + 0.43 C₃H₈O₃ + 1.54CO₂ + 1.3C₂H₅OH + 0.036H₂O 

and determine the product yield coefficient Y etoh/s and determine coefficient a.

TLS Online TPP Program

#Question id: 19487

#Section 4: Fundamentals of Biological Engineering

What is the yield coefficient if the initial substrate concentration is 20 g/l and biomass is 0.2 g/l. The substrate is then consumed and produces 6.0 g/l of biomass?

TLS Online TPP Program

#Question id: 19488

#Section 4: Fundamentals of Biological Engineering

What is the yield coefficient if the initial substrate concentration is 10 g/l and biomass is 0.1 g/l. The substrate is then consumed and produces 5.3 g/l of biomass?

TLS Online TPP Program

#Question id: 19489

#Section 4: Fundamentals of Biological Engineering

The yield coefficient is not used in growth kinetic relationship of which of the following growth kinetics?

TLS Online TPP Program

#Question id: 19530

#Section 4: Fundamentals of Biological Engineering

If air consists of 77% by weight of nitrogen and 23% by weight of oxygen calculate:

(i) the mean molecular weight of air,

(ii) the mole fraction of oxygen,

(iii) the concentration of oxygen in mole/m3 and kg/m3  if the total pressure is 1.5 atmospheres and the temperature is 25 °C.

TLS Online TPP Program

#Question id: 19531

#Section 4: Fundamentals of Biological Engineering

According to the Balances on steady-state processes, the accumulation is equal to?