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TLS Online TPP Program

#Question id: 3527

Which of the following genetic cross would produce 1/4 yellow round, 1/4 yellow wrinkle, 1/4 Green round and ¼ Green Wrinkle seeded plant?

#Section 3: Genetics, Cellular and Molecular Biology

  1. YyRr  X  Yyrr  

  2. Yyrr   X yy Rr

  3. YYrr  X yyRR           

  4. YyRr  X YyRr

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TLS Online TPP Program

#Question id: 20593

#Section 5: Bioprocess Engineering and Process Biotechnology

Which one of the following constants is represented by Kp?
TLS Online TPP Program

#Question id: 27366

#Section 3: Genetics, Cellular and Molecular Biology

If the siRNA is a complete homolog of the target mRNA sequences what is the net result?
TLS Online TPP Program

#Question id: 13056

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
 Which of the following is true for traditional and real time PCR?
TLS Online TPP Program

#Question id: 13038

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order
i. incubate with antibody-enzyme complex that binds primary antibody
ii. coat surface with antigen, block unoccupied sites with nonspecific protein
iii. add substrate, formation of colored product indicates presence of specific antigen
iv. incubate with primary antibody against specific antigen
TLS Online TPP Program

#Question id: 14324

#Section 5: Bioprocess Engineering and Process Biotechnology

Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield of biomass from glucose is measured at 0.48 g g-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g- l, how much change in oxygen requirement for wild-type E. coli unable to synthesise recombinant protein?