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TLS Online TPP Program

#Question id: 13108

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 
 Why is it that some people show three bands?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. The more repeats you have, the longer your DNA fragment is, and longer DNA fragments migrate more slowly in gels.
  2. They are heterozygotes at this region.
  3. They are homozygotes at this region.
  4. They are heterozygotes, and possess one allele that can be cut and one allele that cannot be cut.
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TLS Online TPP Program

#Question id: 14737

#Section 2: General Biology

Which structures are involved in formation of biofilms
TLS Online TPP Program

#Question id: 14741

#Section 5: Bioprocess Engineering and Process Biotechnology

Microbial cell recovery is carried out in a continuous disc-stack centrifuge. The centrifuge is operated at 5000 rpm for separation of baker's yeast. At a feed rate of 60 1/min, 50% of the cells are recovered. At constant speed, solid recovery is inversely proportional to flow rate. What operating speed is required to recover 90% of cells if the flow rate in the centrifuge is maintained at 601/min? 
TLS Online TPP Program

#Question id: 14742

#Section 5: Bioprocess Engineering and Process Biotechnology

Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-4m3, Wr = 3x10-3m3 /kg dry sephacryl, ps =1.25 x 103 kg/m3 . The partition coefficients are kpA =0.38 and kpB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone? 
TLS Online TPP Program

#Question id: 14743

#Section 5: Bioprocess Engineering and Process Biotechnology

for the fermenter with the given data. 
Air is sparged with 0.4 vvm, it is equipped with two sets of impellers and a flat-blade turbine with four baffles. calculate power, P 
TLS Online TPP Program

#Question id: 14744

#Section 5: Bioprocess Engineering and Process Biotechnology

For production of an enzyme used for synthesis of a sun protection lotion, the required kinetic data and constants are: 
vmax = 2.3 mmol.m-3 s-1 Km = 8.9 mmol.-1 So = 12 mmol1- 
Find the reaction time for 95% conversion of raw material 
TLS Online TPP Program

#Question id: 14745

#Section 5: Bioprocess Engineering and Process Biotechnology

heat exchanger, one transfer unit means