Correct statements about RAPD’s

A. RAPD polymorphism is detected by using oligonucleotides usually more than 10 bases long of random sequences as primers in a reaction.

B. In a strain which has in genomic DNA sequences complementary to the primer oligonucleotide, PCR products will be detected in the gel,

C. Typical RAPD markers show limited variation between parents, especially in naturally inbreeding species.

D. RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible.

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#Question id: 14134

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PAM stands for ___

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#Question id: 14135

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is correct about PAM matrix? 

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#Question id: 14136

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is incorrect about global alignment? 

(A) Global alignment is based on the Needleman–Wunsch algorithm.

(B) In Global alignment algorithm, an optimal alignment is obtained over the entire lengths of the two sequences.

(C) It is only suitable for aligning two closely related sequences that are of the same length.

(D) GAP is a web-based pairwise global alignment program.

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#Question id: 14137

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Consider the following alignment of two DNA sequences:

                            AGTAAC

                            AA_ _AC

Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment is _________

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#Question id: 14138

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

BLAST uses heuristics to align a query sequence with all sequences in a database. 

Reason(R): BLASTX uses protein sequences as queries to search against a protein sequence database.

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#Question id: 14139

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is incorrect about sequence similarity and sequence identity?